A JOHN WILEY & SONS, INC. , PUBLICATION, 2003. – 230 p.
This book is about understanding the principles of enzyme kinetics and knowing how to use mathematical models to describe the catalytic function of an enzyme. Coverage of the material is by no means exhaustive. There exist many books on enzyme kinetics that offer thorough, in-depth treatises of the subject. This book stresses understanding and practicality, and is not meant to replace, but rather to complement, authoritative treatises on the subject such as Segel’s Enzyme Kinetics. This book starts with a review of the tools and techniques used in kinetic analysis, followed by a short chapter entitled How Do EnzymesWork? , embodying the philosophy of the book. Characterization of enzyme activity; reversible and irreversible inhibition; pH effects on enzyme activity; multisubstrate, immobilized, interfacial, and allosteric enzyme kinetics; transient phases of enzymatic reactions; and enzyme stability are covered in tu. In each chapter, models are developed from first principles, assumptions stated and discussed clearly, and applications shown.
The treatment of enzyme kinetics in this book is radically different from the traditional way in which this topic is usually covered. In this book, I have tried to stress the understanding of how models are arrived at, what their limitations are, and how they can be used in a practical fashion to analyze enzyme kinetic data. With the advent of computers, linear transformations of models have become unnecessary—this book does away with linear transformations of enzyme kinetic models, stressing the use of nonlinear regression techniques. Linear transformations are not required to carry out analysis of enzyme kinetic data. In this book, I develop new ways of analyzing kinetic data, particularly in the study of pH effects on catalytic activity and multisubstrate enzymes. Since a large proportion of traditional enzyme kinetics used to deal with linearization of data, removing these has both decreased the amount of information that must be acquired and allowed for the development of a deeper understanding of the models used. This, in tu, will increase the efficacy of their use.
The book is relatively short and concise, yet complete.
This book is about understanding the principles of enzyme kinetics and knowing how to use mathematical models to describe the catalytic function of an enzyme. Coverage of the material is by no means exhaustive. There exist many books on enzyme kinetics that offer thorough, in-depth treatises of the subject. This book stresses understanding and practicality, and is not meant to replace, but rather to complement, authoritative treatises on the subject such as Segel’s Enzyme Kinetics. This book starts with a review of the tools and techniques used in kinetic analysis, followed by a short chapter entitled How Do EnzymesWork? , embodying the philosophy of the book. Characterization of enzyme activity; reversible and irreversible inhibition; pH effects on enzyme activity; multisubstrate, immobilized, interfacial, and allosteric enzyme kinetics; transient phases of enzymatic reactions; and enzyme stability are covered in tu. In each chapter, models are developed from first principles, assumptions stated and discussed clearly, and applications shown.
The treatment of enzyme kinetics in this book is radically different from the traditional way in which this topic is usually covered. In this book, I have tried to stress the understanding of how models are arrived at, what their limitations are, and how they can be used in a practical fashion to analyze enzyme kinetic data. With the advent of computers, linear transformations of models have become unnecessary—this book does away with linear transformations of enzyme kinetic models, stressing the use of nonlinear regression techniques. Linear transformations are not required to carry out analysis of enzyme kinetic data. In this book, I develop new ways of analyzing kinetic data, particularly in the study of pH effects on catalytic activity and multisubstrate enzymes. Since a large proportion of traditional enzyme kinetics used to deal with linearization of data, removing these has both decreased the amount of information that must be acquired and allowed for the development of a deeper understanding of the models used. This, in tu, will increase the efficacy of their use.
The book is relatively short and concise, yet complete.