336 Y. Tanaka et al.
and physical stresses. For this integrated system to be robust we have to develop
improved detection systems for modified proteins in proteomics and increase the
number of detectable metabolites in metabolomics. These studies are currently
underway.
References
Agrawal G.K., Jwa N.S., Iwahashi Y., Yonekura M., Iwahashi H., and Rakwal. R. (2006),
Rejuvenating rice proteomics: Facts, challenges, and visions. Proteomics, 6, 5549–5576.
Celemedson C., et al. (44Scientists) (1996), MEIC Evaluation of acute systemic toxicology. ALTA,
24, 252–272.
Fujita K., Matsuyama A., Kobayashi K., and Iwahashi H. (2004),Comprehensive gene expression
analysis of the response to straight-chain alcohols in Saccharomyces cerevisiae using cDNA
microarray. J. Appl. Microbiol., 97, 57–67.
Iwahashi H. (2006), Yeast genes that star in the cross protection against environmental stress,
Cryobiol. Cryotech., 52, 55–59.
Iwahashi H. (2000), Multiple-end-point bioassay using microorganisms. Biotech. Biop. Eng.,
5, 400–406.
Iwahashi H., Yang W., and Tanguay R.M. (1995), Detection and expression of the 70kDa heat
shock protein ssbp1 at different temperature in the yeast Saccharomyces cerevisiae. Biochem.
Biophys. Res. Comm., 213, 484–489.
Iwahashi H., Shimizu H., Odani M., and Komatsu Y. (2003), Piezophysiology of genome wide
gene expression levels in the yeast Saccharomyces cerevisiae. Extremophile, 7, 291–298.
Iwahashi H., Odani M., Ishidou E., and Kitagawa E. (2005), Adaptation of Saccharomyces cerevisiae
to high hydrostatic pressure causing growth inhibition FEBS Lett., 579, 2847–2852.
Jwa N.S., Agrawal G.K., Tomogami M., Yonekura M., Hane O., Iwahashi H., and Rakwal. R.
(2006), Defense/Stress-related marker genes, proteins and secondary metabolites in defining rice
self-defense mechanisms, Plant Physiol. Biochem., 44, 261–273.
Kersten B., Agrawal G.K., Iwahashi H, and Rakwal R. (2006), Plant phosphoproteomics: A long
road ahead. Proteomics., 6, 5517–5528.
Kim H., Ishidou E., Kitagawa E., Momose Y., and Iwahashi H. (2004), Yeast DNA microarray for
the evaluation of the toxicity in environmental water containing the burned ash. Env. Monit.
Assess., 92, 253–272.
Kimura S., Ishidou E., Kurita S., Suzuki Y., Shibato J., Rakwal, R., and Iwahashi H. (2006), DNA
microarray analyses reveal a post-irradiation differential time-dependent gene expression pro-
file in yeast cells exposed to X-rays and γ-rays. Biochem. Biophys. Res. Comm., 346, 51–60.
Kitagawa E., Takahashi J., Momose Y., and Iwahashi H. (2002), The effects of the pesticide
thiuram: Genome-wide screening of indicator genes by yeast DNA microarray. Environ. Sci.
Technol., 36, 3908–3915.
Kitagawa E., Momose Y., and Iwahashi H. (2003), Correlation of the structures of agricultural
fungicides to gene expression in Saccharomyces cerevisiae upon exposure to toxic doses.
Environ. Sci. Technol., 15, 2788–2793.
Kurita S., Kitagawa E., Kim C.H., Momose Y., and Iwahashi H. (2002), Studies on the antimicrobial
mechanisms of capsaicin using yeast DNA microarray. Bios. Biotechnol. Biochem., 66, 532–536.
Kurita S., Shirisatta S., Kitagawa E., Momose Y., Ishigami Y., and Iwahashi H. (2004), New
methods for the assessment of biological effect by surfactants using yeast DNA microarray.
J. Oleo Sci., 53, 387–398.
Matsuoka H., Suzuki Y., Iwahashi H., Arao T., Suzuki Y., and Tamura. K.(2005), The biological
effects of high-pressure gas on the yeast transcriptome. Braz. J. Med. Biol. Res., 38, 1267–72.