31 In Vitro Testing of Inhalable Fly Ash at the Air Liquid Interface 413
treatment of the particles such as deposition on filters and resuspension. The results
of this study show that an in vitro exposure of pulmonary cells at the air-liquid
interface can be conducted by the way described under methods without loss of cell
viability. That means transport and exposure to low flow rates has no effect on the
viability of the cells.
This exposure technique can therefore be used for screening the toxicological
potential of unknown aerosols in order to identify potential emitters of health
relevant particles. Furthermore, new developments in the technology of the
combustion process and flue gas cleaning can directly be evaluated.
Acknowledgements We thank H. Fischer for taking the SEM images, F. Seidenstricker and M.
Hauser for useful help in the laboratory. The financial support of BWPLUS (Baden-Württemberg
Research Program Securing a Sustainable Living Environment) is appreciated.
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