InTech. 2011. 336 p.
The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.
Contents
Technological Advances
Screening of Bacterial Recombinants: Strategies and Preventing False Positives
Non-Viral Vehicles: Principles, Applications, and Challenges in Gene Delivery
Cancer and Cell Biology
Subcloning and Expression of Functional Human Cathepsin B and K in E. coli: Characterization and Inhibition by Flavonoids
Molecular Cloning and Overexpression of WAP Domain of Anosmin-1 (a-WAP) in Escherichia coli
Effects of Two Novel Peptides from Skin of Lithobates Catesbeianus on Tumor Cell Morphology and Proliferation
Immunology/Hematology
Molecular Cloning of Immunoglobulin Heavy Chain Gene Translocations by Long Distance Inverse PCR
Identification of Molecules Involved in the Vulture Immune Sensing of Pathogens by Molecular Cloning
Molecular Cloning, Characterization, Expression Analysis and Chromosomal Localization of the Gene Coding for the Porcine ?IIb Subunit of the ?IIb?3 Integrin Platelet Receptor
Molecular Cloning, Expression, Purification and Immunological Characterization of Proteins Encoded by Regions of Difference Genes of Mycobacterium tuberculosis
Toxicology
Molecular Toxinology – Cloning Toxin Genes for Addressing Functional Analysis and Disclosure Drug Leads
Molecular Cloning, Expression, Function, Structure and Immunoreactivities of a Sphingomyelinase D from Loxosceles adelaida, a Brazilian Brown Spider from Karstic Areas
Parasitology
Cloning the Ribokinase of Kinetoplastidae: Leishmania Major
Genome Based Vaccines Against Parasites
Phosphagen Kinase System of the Trematode Paragonimus westermani: Cloning and Expression of a Novel Chemotherapeutic Target
Evolutionary Biology
Molecular Cloning, Expression Patte, and Phylogenetic Analysis of the Lysyl-tRNA Synthetase Gene from the Chinese Oak Silkworm Antheraea peyi
Molecular Cloning and Characterization of Fe-Superoxide Dismutase (Fe-SOD) from the Fe Ceratopteris thalictroides
Plant Biology
Cloning and Characterization of a Candidate Gene from the Medicinal Plant Catharanthus roseus Through Transient Expression in Mesophyll Protoplasts
Positional Cloning in Brassica napus: Strategies for Circumventing Genome Complexity in a Polyploid Plant
The development of molecular cloning technology in the early 1970s created a revolution in the biological and biomedical sciences that extends to this day. The contributions in this book provide the reader with a perspective on how pervasive the applications of molecular cloning have become. The contributions are organized in sections based on application, and range from cancer biology and immunology to plant and evolutionary biology. The chapters also cover a wide range of technical approaches, such as positional cloning and cutting edge tools for recombinant protein expression. This book should appeal to many researchers, who should find its information useful for advancing their fields.
Contents
Technological Advances
Screening of Bacterial Recombinants: Strategies and Preventing False Positives
Non-Viral Vehicles: Principles, Applications, and Challenges in Gene Delivery
Cancer and Cell Biology
Subcloning and Expression of Functional Human Cathepsin B and K in E. coli: Characterization and Inhibition by Flavonoids
Molecular Cloning and Overexpression of WAP Domain of Anosmin-1 (a-WAP) in Escherichia coli
Effects of Two Novel Peptides from Skin of Lithobates Catesbeianus on Tumor Cell Morphology and Proliferation
Immunology/Hematology
Molecular Cloning of Immunoglobulin Heavy Chain Gene Translocations by Long Distance Inverse PCR
Identification of Molecules Involved in the Vulture Immune Sensing of Pathogens by Molecular Cloning
Molecular Cloning, Characterization, Expression Analysis and Chromosomal Localization of the Gene Coding for the Porcine ?IIb Subunit of the ?IIb?3 Integrin Platelet Receptor
Molecular Cloning, Expression, Purification and Immunological Characterization of Proteins Encoded by Regions of Difference Genes of Mycobacterium tuberculosis
Toxicology
Molecular Toxinology – Cloning Toxin Genes for Addressing Functional Analysis and Disclosure Drug Leads
Molecular Cloning, Expression, Function, Structure and Immunoreactivities of a Sphingomyelinase D from Loxosceles adelaida, a Brazilian Brown Spider from Karstic Areas
Parasitology
Cloning the Ribokinase of Kinetoplastidae: Leishmania Major
Genome Based Vaccines Against Parasites
Phosphagen Kinase System of the Trematode Paragonimus westermani: Cloning and Expression of a Novel Chemotherapeutic Target
Evolutionary Biology
Molecular Cloning, Expression Patte, and Phylogenetic Analysis of the Lysyl-tRNA Synthetase Gene from the Chinese Oak Silkworm Antheraea peyi
Molecular Cloning and Characterization of Fe-Superoxide Dismutase (Fe-SOD) from the Fe Ceratopteris thalictroides
Plant Biology
Cloning and Characterization of a Candidate Gene from the Medicinal Plant Catharanthus roseus Through Transient Expression in Mesophyll Protoplasts
Positional Cloning in Brassica napus: Strategies for Circumventing Genome Complexity in a Polyploid Plant