Oxford University Press, 2000. - 368 p. ISBN: 0199638128
Univ. of Southampton, UK. This book describes the theory and practical applications of many analytical techniques based upon the light absorbing properties of molecules. Such techniques form the basis of routine tests in chemical, biological, and medical science laboratories. Practical advice is given, from experts in the different topics, together with lists of useful literature. Contents
List of protocols
Abbreviations
Introduction to light absorption; visible and ultraviolet spectra
Fluorescence principles and measurement
Time resolved fluorescence and phosphorescence spectroscopy
An introduction to circular dichroism
Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods
Steady state kinetics
Spectrophotometric assays
Stopped flow spectroscopy
Stopped flow fluorescence spectroscopy
Stopped flow circular dichroism
Spectrophotometry and fluorimetry of cellular compartments and intracellular processes
Use of optical spectroscopic methods to study the thermodynamic stability of proteins
The use of spectroscopic techniques in the study of DNA stability
Univ. of Southampton, UK. This book describes the theory and practical applications of many analytical techniques based upon the light absorbing properties of molecules. Such techniques form the basis of routine tests in chemical, biological, and medical science laboratories. Practical advice is given, from experts in the different topics, together with lists of useful literature. Contents
List of protocols
Abbreviations
Introduction to light absorption; visible and ultraviolet spectra
Fluorescence principles and measurement
Time resolved fluorescence and phosphorescence spectroscopy
An introduction to circular dichroism
Quantitative determination of equilibrium binding isotherms for the multiple ligand - macromolecule interactions using spectroscopic methods
Steady state kinetics
Spectrophotometric assays
Stopped flow spectroscopy
Stopped flow fluorescence spectroscopy
Stopped flow circular dichroism
Spectrophotometry and fluorimetry of cellular compartments and intracellular processes
Use of optical spectroscopic methods to study the thermodynamic stability of proteins
The use of spectroscopic techniques in the study of DNA stability