2nd ed. BIOS Scientific Publishers. 1997. 157 p.
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope.
Contents
Introduction
Production of Antibodies
Requirements for Immunocytochemistry
Colour plates
Methods
Specificity Problems and Essential Controls
Enhancements of Standard Methods
Multiple Immunostaining
Post-embedding Immunocytochemistry for the Transmission Electron Microscope
In Vitro Methods for Testing Antigen-Antibody Reactions
Applications of Immunocytochemistry
Microscopy
Appendix: Technical Notes
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope.
Contents
Introduction
Production of Antibodies
Requirements for Immunocytochemistry
Colour plates
Methods
Specificity Problems and Essential Controls
Enhancements of Standard Methods
Multiple Immunostaining
Post-embedding Immunocytochemistry for the Transmission Electron Microscope
In Vitro Methods for Testing Antigen-Antibody Reactions
Applications of Immunocytochemistry
Microscopy
Appendix: Technical Notes