The microorganisms utilize the absorbed organic matter as a carbon
and energy source for cell growth and convert it to cell tissue, water, and
oxidized products (mainly carbon dioxide, CO
2
). Some bacteria attack
the original complex substance to produce simple compounds as their
waste products. Other bacteria then use these waste products to produce
simpler compounds until the food is used up.
The mixture of wastewater and activated sludge in the aeration basis
is called mixed liquor. The biological mass (biomass) in the mixed liquor
is called the mixed liquor suspended solids (MLSS) or mixed liquor
volatile suspended solids (MLVSS). The MLSS consists mostly of
microorganisms, nonbiodegradable suspended organic matter, and other
inert suspended matter. The microorganisms in MLSS are composed of
70% to 90% organic and 10% to 30% inorganic matter (Okun, 1949;
WEF and ASCE, 1996a). The types of bacterial cell vary, depending on
the chemical characteristics of the influent waste-water tank conditions
and the specific characteristics of the microorganisms in the flocs.
Microbial growth in the mixed liquor is maintained in the declining or
endogenous growth phase to insure good settling properties.
After a certain reaction time (4 to 8 h), the mixed liquor is discharged
from the aeration tank to a secondary sedimentation basin (settling
tank, clarifier) where the suspended solids are settled out from the
treated wastewater by gravity. However, in a sequencing batch reactor
(SBR), mixing and aeration in the aeration tank are stopped for a time
interval to allow MLSS to settle and to decant the treated wastewater;
thus a secondary clarifier is not needed in an SBR system. Most con-
centrated biological settled sludge is recycled back to the aeration tank
(so-called return activated sludge, RAS) to maintain a high population
of microorganisms to achieve rapid breakdown of the organics in the
wastewater. The volume of RAS is typically 20% to 30% of the wastewater
flow. Usually more activated sludge is produced than return sludge.
The treated wastewater is commonly chlorinated and dechlorinated,
then discharged to receiving water or to a tertiary treatment system
(Fig. 6.3). The preliminary, primary, and activated-sludge (biological)
processes are included in the so-called secondary treatment process.
21.1 Aeration periods and BOD loadings
The empirical design of activated sludge is based on BOD loading, food-
to-microorganism ratio (F/M), sludge age, and aeration period. Empirical
design concepts are still acceptable. The Ten States Standards states
that when activated-sludge process design calculations are not submit-
ted, the aeration tank capacities and permissible loadings for the sev-
eral adoptions of the processes shown in Table 6.11 are used as simple
design criteria. Those values apply to plants receiving diurnal load
622 Chapter 6