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Vlak J.M., de Gooijer C.D., Tramper J., Miltenburger H.G. (Eds.) Insect Cell Cultures: Fundamental and Applied Aspects
Kluwer Academic Publishers, 2002, 316 pages

Insect Cell Cultures: Fundamental and Applied Aspects is a comprehensive reference work, covering the key issues involved in nearly 30 review papers: cell lines (development, characterisation, physiology, cultivation and medium design); viruses (virus-cell interactions, replication, recombinant construction, infection kinetics, post-translational modification and passage effects); engineering (shear, bioreactors including perfusion, immobilisation, scale-up and modelling, downstream processing); applications; economics and regulatory aspects.
For cell biologists, biochemists, molecular biologists, virologists, immunologists and other basic and applied disciplines related to cell culture engineering, both academic and industrial.
Insect cell cultures fundamental and applied aspects provides a comprehensive review of the major advances in insect cell culture research. The book is divided into five sections which systematically approach the topic, from the basic principles of cell culture technology through the concomitant economic and regulatory issues that are important aspects of the commercialization of bio-based products. All chapters in this volume are written by the foremost authorities in the field who have contributed original research in insect cell and molecular biology, chemical engineering, and baculovirology. The first part is devoted to an in-depth review of basic insect cell culture and cell biology with chapters on new advances in transgenic cells and novel media formulations. Part two presents an exceptionally lucid survey of the molecular biology of baculoviruses and their development as expression vectors. These introductory virus chapters are followed by specialized topics on the expression of recombinant proteins and their posttranslational modification. Part three, devoted to review chapters on the application of bioengineering principles for the scale-up of insect cell culture, also discusses the differences between invertebrate and vertebrate cell culture and the impact of cell bioreactor design, shear force conces, nutrient and oxygen requirements that are specific for insect cells. Parts four and five detail examples of specific commercial products produced in insect cell systems. The book concludes with an exhaustive consideration of the economic and regulatory issues that have to be considered by industry and govement agencies. The topics discussed are interrelated through a focus on the insect cell culture-baculovirus system and the prominent role it plays in basic biological research and biotechnology.
The book benefits from clear and precise writing throughout. All chapters are well documented with figures, illustrations, and comprehensive bibliographies. This authoritative volume will serve as a standard information resource on insect cell culture. The book will become the benchmark reference manual for students and researchers in academia and govement laboratories requiring information on how insect cell systems are being applied in basic research and biotechnology applications. In particular this treatise will be an invaluable source of knowledge
to industrial scientists who are applying the baculovirus-cell culture systems for commercial purposes.
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