
starter cultures in small cavities has significant impli-
cations on the entire fermentation and on the inacti-
vation of pathogens, which are often located in
distant areas of the food matrix. An even distribution
of bacteria in the sausage is important for optimizing
the shelf-life of fermented sausages, obtained by
adequately mixing the sausage ingredients and using
liquid starter cultures.
Some Typical Examples on the Market
0020 Pet foods have been on the market since the 1960s;
they are processed by blending and pasteurizing meat
byproducts, cereals, and other ingredients including
humectants (sucrose and propylene glycol), followed
by extrusion cooking techniques to form a variety
of products. Their shelf-life stability of more than
12 months is based on reduced water activity
(a
w
0.85), heat processing, and addition of anti-
mycotics, most commonly potassium sorbate. Other
ingredients, such as sodium caseinate and starch, are
sometimes added for specific textural characteristics.
0021 There are many different traditional IM meats.
One example is salami, which is a fermented sausage
originating from Europe. Its shelf-life stability depends
on a specific sequence of the stabilizing factors pre-
servatives, redox potential, competitive microflora,
acidification, and a
w
. Early in sausage ripening,
nitrite added with curing salts inhibits the growth of
many bacteria, such as Salmonella spp. Nitrite levels
eventually decrease, but other bacteria continue to
multiply and cause a reduction in redox potential.
This inhibits the growth of aerobic organisms, while
favoring lactic acid bacteria, which produce acid
and reduce the pH of sausages. In long-ripened fer-
mented sausages, a
w
(0.90) becomes the main sta-
bilizing factor, as numbers of lactic acid bacteria
decrease with storage time, resulting in slight in-
creases in acidification and redox potential. Other
fermented IM meats are French saucisson, and
Turkish pastirma.
0022 Beef jerky or jerked beef (North America), lup
cheong (China), biltong (South Africa), and carne de
sol (South America) are examples of nonfermented
IM meats which are produced in different regions of
the world. Various combinations of factors contribute
to product stability, such as a
w
reduction by addition
of salt and sugar, mild heat treatments, vacuum pack-
aging, and preservatives. A variety of other ingredi-
ents, such as vinegar, pepper, coriander, and garlic,
are used for flavor and shelf-life improvements in IM
meats.
0023 IM fruit pastes and pure
´
es, intended for use in
candy, cookies, icecream, etc., have been developed
by treating various fruits (apricots, peaches, papayas,
etc.) with sulfur dioxide, dehydrating to a
w
0.70
and employing extrusion techniques. Meal replace-
ment bars (a
w
of approximately 0.60) are IMF
which contain a variety of ingredients such as milk
protein, soya protein, fat, flour, starch, sucrose, salt,
propylene glycol, and preservatives. Multitextured
shelf-stable products have been introduced as well,
including moist puddings in e
´
clair-type shells; ready-
to-eat breakfast cereals containing dried fruits; snack
bars with fruit fillings; and cookies with crunchy
exteriors and soft-moist interiors. Components of
multitextured foods initially have different a
w
and
equilibrate to a common a
w
during storage.
Factors Limiting IMF Stability
0024The significant effects of a
w
on chemical reactions
have important implications for the preservation
of IMF. The shelf-life of IMF can be limited by
nonenzymatic browning reactions, particularly the
Maillard reaction which is a condensation reaction
between the carbonyl group of a reducing compound,
mainly sugar, and a free-amino group. The reaction
can greatly affect food color due to the formation of
brown pigments during processing and storage. It is
also associated with toughening of texture, decreases
in protein quality, and slight increases in a
w
. Inversely,
certain Maillard reaction products have bactericidal
effects which contribute to product stability. (See
Browning: Nonenzymatic; Storage Stability: Param-
eters Affecting Storage Stability.)
0025Generally, as for most deteriorative reactions,
browning reaction rates increase exponentially with
increasing a
w
above the food monolayer due to an
increased mobility of reactants (Figure 1). Maximum
browning rates occur at approximately 0.65–0.75,
which is in the IMF a
w
range, and then the reaction
decreases as a result of reactant dilution and product
inhibition.
0026Variations in browning rate maxima are due to
differences in the physical and chemical structure of
IMF. Liquid humectants, such as glycerol or propylene
glycol, reduce browning in IMF by shifting a
w
maximas
from approximately 0.7 to 0.4–0.5. These humectants
appear to act as aqueous solvents, allowing greater
reactant mobility at lower moisture contents. Maillard
reaction rates also decrease with decreasing pH and the
addition of sulfur dioxide or sulfites, which are used in
IMF as browning inhibitors.
0027Free radical autoxidation of unsaturated lipids, and
other compounds, is another important shelf-life
problem of IMF. In the IMF a
w
range, lipid oxidation
rates generally increase with increasing water content
(Figure 1) due to greater mobility of reactants, dissol-
ution of precipitated catalysts, and swelling of solid
3340 INTERMEDIATE-MOISTURE FOODS